Application of high-throughput sequencing to answer basic biological questions
on RNA polymerase II activity
Sing-Hoi Sze, TAMU
Time: 9:00am Monday March 18 2024 {US Central daylight saving time}
Abstract:
What mechanisms are involved in transcription? Which are the active residues of the transcription machinery? How to determine where transcription initiates among multiple start sites? These are biological questions that can be addressed by high-throughput sequencing on transcription, which is carried out by the RNA polymerase. By synthesizing a large set of mutations in parallel within a conserved region of RNA polymerase II called the trigger loop, the effects of single and double mutants are examined. By generating promoter sequences genome-wide, start site preferences are assessed with respect to the promoter scanning model. This work is in collaboration with Craig Kaplan, whose lab utilizes yeast as a model to understand gene regulation.
Sing-Hoi Sze:
Sing-Hoi Sze is an associate professor of computer science and engineering and of biochemistry & biophysics at Texas A&M University. Before joining Texas A&M, he was a postdoctoral research associate at the University of California, San Diego. He received his Ph.D. in computer science from the University of Southern California. His current research is on algorithms for analyzing high-throughput sequencing data, including transcriptome and microbiome analysis.
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